Online Ultra-High Performance Liquid Chromatography and Probe Based-Sampling
Online HPLC analysis is becoming increasingly important for studying the kinetic profiles of chemical reactions, from which valuable mechanistic information can be obtained. Historically, online sample collection was performed via either flow of the reaction medium through a sample loop, followed by injection, or by metered withdrawal of a defined volume flowing via a sampling tube or capillary. Both of these approaches work well for homogeneous solutions, but are not well suited to the investigation of heterogeneous reactions where solids are present or where active precipitation or crystallization is taking place.
ETC is seeking companies interested in supplying a vendor-supported online sampling system with integrated liquid chromatographic (LC) analysis employing a low volume, probe-based sampling system capable of repeated automated sampling with the ability to perform direct sample injection for LC analysis for any desired number of reaction time points. Automated sampling is expected to accommodate both homogeneous and heterogeneous (solid/liquid) reaction mixtures, and in the case of heterogeneous mixtures, the sampler is expected to perform representative sampling of both the solid and liquid components. Also, a cart-mounted system to allow flexibility and portability in the laboratory environment is required. Lastly, a fully integrated data management and data visualization environment is also deemed critical, enabling seamless collation of sampling specifics and chromatographic results for further analysis.
Any questions received will be anonymized and made available to all respondents via our website.
RFI issued December 1, 2016
Questions Due December 8, 2016
Responses due December 23, 2016
Questions Received (updated December 6, 2016)
- Can you clarify what is meant by, “Both of these approaches work well for homogeneous solutions, but are not well suited to the investigation of heterogeneous reactions where solids are present or where active precipitation or crystallization is taking place “. Does this mean the probe will filter out the solids/crystals from the process stream or does the probe need a mechanism for sampling solids? The ideal sampling system would be capable of either sampling strategy (liquid only vs. a liquid+solid sample.) In fact, the table in section 5.2 of the RFI lists a representative (solid+liquid) sample, as a required functional requirement, and a filtered sample (liquid only) as a desired functional requirement.